Shodex F7009000 RSpak RP18-415 Polymeric Reversed Phase HPLC Columns, 6µm , 150mm, 4.6mm

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Product Acetonitrile0 to 100%
Product In-column solventH₂O/CH₃CN (5/95)
Product Max. Pressure (MPa)22
Product Max. Usable Flow Rate (mL/min)1.5
Product Methanol0 to 100%
Product Particle size, um6
Product pH range1 to 13
Product Plate number (TP/Column)>5,000
Product Pore Size Average (Å)450
Product Salt conc. (M)< 0.5
Product Size (I.D. x Length, mm)4.6 x 150
Product Temperature Range (°C)RT to 50
Product THF0 to 50%
Product Toluene Chloroform etc.0 to 50%
Product Water solution0 to 100%

  • £919.02
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Reversed Phase Chromatography (RP)

  • Separation is based on the partition equilibrium between stationary phase and mobile phase.
  • The polarity of the stationary phase is lower than that of the mobile phase.
  • Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
  • Using the lower polarity mobile phase causes a faster elution.

RSpak RP18

  • Large pore size is suitable for the analysis of proteins and peptides (biomolecules)
  • Corresponds USP LP21

Standard Proteins (3) (RP18-415)

RSpak RP18-415 (a column for reversed phase chromatography) can analyze a variety of proteins with good recovery. This column is suitable for fast analysis at high flow rates since the maximum working pressure is 22Mpa.

Recovery of Proteins
1. Ribonuclease A93%
2. Insulin98%
3. Cytochrome c100%
4. Lysozyme100%
5. BSA98%
6. Myoglobin108%
7. Ovalbumin---
Column       : Shodex RSpak RP18-415 (4.6mmI.D. x 150mm)

Eluent       : (A); 0.1% TFA aq./CH3CN=99/1                (B); 0.1% TFA aq./CH3CN=5/95                Linear gradient: 0 to 25min, 20% (B) to 60% (B)

Flow rate    : 1.0mL/min

Detector     : UV(220nm)

Column temp. : Room temp.
    • SKU
  • Manufacturer
    Showa Denko Europe GmbH
  • Chromatography HPLC or GC
    • Particle size (µm)
    • Internal Diameter (mm)
    • Length (mm)
    • Temperature range (°C)
      20 to 50
    • pH Range
      1 to 13
    • Packing Phase
      RSpak RP18-415