Shodex F6989201 High Performance Semi-micro Aqueous SEC (GFC) Columns, PROTEIN KW402.5-4F, Particle size (µm) 3, Column Length 300mm, 4.6mm Internal Diameter
Usable organic solvent concentration: Up to 100% for methanol and acetonitrile.
KW400 series is recommended to be used with semi-micro type devices.
Specification
| Product Exclusion limit (Proteins) | 150,000 |
| Product Exclusion limit (Pullulan) | 60,000 |
| Product Max. Pressure (MPa) | 10 |
| Product Particle size, um | 3 |
| Product pH range | 3 - 7.5 |
| Product Plate number (TP/Column) | >35,000 |
| Product Pore Size Maximum | 400 |
| Product Size (I.D. x Length, mm) | 4.6 x 300 |
| Product name | Max. usable pressure (MPa) | Recommended flow rate (mL/min) | Max. usable flow rate (mL/min) | Usable temp. range (°C) | Usable pH range | Usable salt conc. |
|---|---|---|---|---|---|---|
| KW402.5-4F KW403-4F KW404-4F KW405-4F KW400G-4A | 10 10 7 7 - | 0.35 0.35 0.35 0.35 - | 0.5 0.5 0.5 0.5 - | 5 to 45 5 to 45 5 to 45 5 to 45 5 to 45 | 3.0 to 7.5 3.0 to 7.5 3.0 to 7.5 3.0 to 7.5 3.0 to 7.5 | ≤ 0.3M ≤ 0.3M ≤ 0.3M ≤ 0.3M ≤ 0.3M |
For analyzing substances using aqueous SEC (GFC) mode, there are two kinds of columns: silica-based columns and polymer-based columns.
Generally, silica gel provides better resolution because the pore size distribution is sharper and the pore volume is larger than polymer-based columns. However, silica-based columns cannot be used with the eluent whose pH is 7.5 or more because silica gel is dissolved in such eluent. In contrast, polymer-based columns have a wider usable eluent pH range and these columns can be washed with alkaline solution, resulting in longer column life.
| KW400 | · Reduced packing material particle size enhances column performance · Three to four-fold higher sensitivity than KW-800 series · KW405-4F is applicable analyzing samples with molecular weight above 1,000,000 · Fulfills USP L20, L33, and L59 requirements |
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(High performance semi-micro columns)
| Product code | Product name | *Plate number (TPN/column) | Particle size (μm) | Size (mm) I.D. x Length | Shipping solvent |
|---|---|---|---|---|---|
| F6989201 F6989202 F6989203 F6989204 F6700132 | KW402.5-4F KW403-4F KW404-4F KW405-4F KW400G-4A | ≥ 35,000 ≥ 35,000 ≥ 25,000 ≥ 25,000 (guard column) | 3 3 5 5 5 | 4.6 x 300 4.6 x 300 4.6 x 300 4.6 x 300 4.6 x 10 | H2O H2O H2O H2O H2O |