Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns
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Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns

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Chiral Technnologies (Part of Daicel Group)
SKU Internal Diameter (mm) Column Length (mm) Particle size (µm) Stationary Phase Type Weight (In Grams) Price (excluding VAT) Availability Qty To Purchase
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33713 4 100 5 CHIRALPAK® CBH Analytical 1000 £1105.00 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33714 4 150 5 CHIRALPAK® CBH Analytical 1000 £1449.25 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33712 4 50 5 CHIRALPAK® CBH Analytical 1000 £871.25 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33783 3 100 5 CHIRALPAK® CBH Analytical 1000 £1105.00 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33784 3 150 5 CHIRALPAK® CBH Analytical 1000 £1449.25 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33782 3 50 5 CHIRALPAK® CBH Analytical 1000 £871.25 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33793 2.1 100 5 CHIRALPAK® CBH Analytical 1000 £1215.50 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33794 2.1 150 5 CHIRALPAK® CBH Analytical 1000 £1589.50 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33792 2.1 50 5 CHIRALPAK® CBH Analytical 1000 £952.00 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33733 10 100 5 CHIRALPAK® CBH Semi-Preparative 1000 £3854.75 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33734 10 150 5 CHIRALPAK® CBH Semi-Preparative 1000 £5605.75 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33781 3 10 5 CHIRALPAK® CBH Guard (Pack of 2) 800 £199.75 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33791 2 10 5 CHIRALPAK® CBH Guard (Pack of 2) 800 £199.75 1 + -
Daicel CHIRALPAK® CBH Cellobiohydrolase Enzyme Protein Based Chiral Columns 33711 4 10 5 CHIRALPAK® CBH Guard (Pack of 2) 800 £229.50 1 + -

CHIRALPAK ® CBH

Silica surface covalently bonded cellobiohydrolase

CBH is a protein-bonded chiral column, the surface of which is covalently bound to cellobiohydrolase.  

Cellobiohydrolase (CBH) is a stable enzyme which has been immobolized onto 5µm spherical silica particles creating the chiral stationary phase in the Chiral-CBH column. This is also a reversed phase column, used for the direct separation of enantiomers. The column is preferentially used for the separation of the enantiomers of basic drugs from many compound classes

We recommend the use of a guard cartridge, in order to protect the analytical column from impurities with high affinity and particulate impurities. The guard cartridge should be replaced regularly; otherwise the column performance can be affected. This is of special importance in bioanalysis.

For compounds of acidic category, however, it is preferred to use CHIRALPAK® AGP and CHIRALPAK® HSA columns 

  • The chiral selector is Cellobiohydrolase (CBH). This is a stable enzyme, which has been immobilized onto 5 micrometer spherical silica particles.
  • CHIRALPAK®CBH is used for the separation of enantiomers of basic drugs from many compound classes. CHIRALPAK®CBH separates preferentially compounds containing one or more basic nitrogen's together with one or more hydrogen accepting or hydrogen donating groups (alcohol, phenol, carbonyl, amide, ether, sulphoxide, ester etc.).

 

Parameters

Filler particle size CHIRALPAK ® CBH: of 5 m
PH PH 2.0 to 9.0
temperature 20 to 30 ° C
The solvent was stored The H 2 O / 2-Propanol = 85/15 (V / V)
Precautions for storage In order to prevent high temperature and lead to protein denaturation, damage to the separation of chiral column capacity, it is recommended to save in the refrigerator (4 ℃)


Suitable for inverting systems.

Scope of application: 

The isolated compounds are preferably primary and secondary amines containing benzene ring structures . It is
not necessary to have hydrogen bonding groups. However, the molecular junction  structure if the hydrogen-bonding groups containing, for example, a carbonyl group , an alcoholic hydroxyl group, amide, sulfoxide groups, which will  result in better enantioselectivity.

Chromatographic Conditions

The mobile phases are buffer solutions with a relatively low content of uncharged organic modifier. Note! Do not use charged organic modifiers in the mobile phase. The mobile phases are mixtures of phosphate or acetate buffers and organic solvents as 2-propanol or acetonitrile. The retention and the enantioselectivity can be regulated by changes in pH, buffer concentration and organic modifier (nature and concentration).

Storage Conditions

The column should be used at room temperature or below. When the column is stored for long periods of time it is recommended to fill with 10% 2-propanol in distilled water and place it in the refrigerator.

Cleaning of the Column

If the column has been contaminated, wash the column over night with 10% 2-propanol in distilled water at a flow rate of 0.2mL/min 

We recommend the use of a guard cartridge, in order to protect the analytical column from impurities with high affinity and particulate impurities. The guard cartridge should be replaced regularly; otherwise the column performance can be affected. This is of special importance in bioanalysis.


Click On The Red Link To Visit - The Chiral Application Search, which  has been developed to help you find the best enantiomeric separation.

By searching structure and application you will be able to access over 2100 chiral separations some of which offer multiple separation conditions . We have included examples of CHIRALCEL® / CHIRALPAK® (Polysaccharide, Protein and Quinine / Quinidine) and CROWNPAK® (Crown ether) columns.

In addition, the application data of certain generic API compounds which are registered with the “USP, EP and JP Pharmacopeia are included alongside suggested alternative conditions found by Daicel scientists.

    • Weight (In Grams)
      1000 g
    • SKU
      33713
    • Commodity Codes
      90279050
  • Manufacturer
    Chiral Technnologies (Part of Daicel Group)
  • Country of Origin
    Japan
  • Internal Diameter (mm)
    2.1, 3, 4, 10, 2
  • Column Length (mm)
    10, 50, 100, 150
  • Particle size (µm)
    5, Not Applicable
  • Stationary Phase
    CHIRALPAK® CBH, Not Applicable
  • Type
    Analytical, Semi-Preparative, Guard (Pack of 2)

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