Daicel Protein-Based Columns

Columns packed with the immobilized protein stationary phases (PSPs) are successfully utilized by scientists for separation and characterization of chiral compounds in a great number of applications, ranging from drug discovery and quality assurance of marketed drugs to environmental monitoring.

The chiral selector for the CHIRALPAK® AGP is α1-acid glycoprotein (AGP), while human serum albumin (HSA) is the chiral selector for CHIRALPAK HSA. The chiral selector for CHIRALPAK CBH is cellobiohydrolase, a stable enzyme. The three chiral selectors are immobilized on 5-µm spherical silica particles. The PSPs function entirely in the reversed-phase chromatographic mode, using buffers with low organic modifier content and at moderate pH values. Enantioselectivity can easily be controlled or improved by changes in the mobile phase composition: pH, buffer and organic modifier types and concentrations.

All PSP selectors are packed in columns with 3-, 4- and 10-mm internal diameter to cover separations on both analytical and semi-preparative scales.