L-column Micro is a nano/micro column with 0.075–0.3 mm I.D. It is a high-performance column that was manufactured by combining low-absorption packing materials, including L-column2 ODS, an originally developed packing technique and a column structure with a small dead volume.

Features 　　　

• The packing materials can be selected from L-column series including L-column2 ODS.
• Highly sensitive analyses are possible because of the negligible adsorption of basic peptides and proteins. 
• Stabilization time can be shortened and highly sensitive analyses are possible because of low column bleed. 
• An inert fused silica capillary is used as the column body, with two kinds of column structures available.

Proteome analysis 

BSA tryptic digest was analyzed using LC/MS/MS and the sample concentration and cover ratio was determined using L-column Micro and another brand. A higher cover ratio means that more amino acid sequences are read. L-column Micro shows advantage at all concentration levels, but excels at the lower levels . With superior end-capping and very high theoretical plate numbers per column, L-column Micro permits identification of many proteins and is the optimum choice for proteome analysis 

Low adsorption and high efficiency 

L-column Micro can separate many peptides because of the low-adsorption packing materials. This is effective for the identification of proteins. The column has a high theoretical plate number and high durability because it is packed homogeneously by a patented technique.

Trap column

In proteomic analysis using LC/MS/MS, the trap column is indispensable for increasing the injection volume. A small dead volume, low-adsorption property and high retention ability that traps target constituents are required effectively in a trap column.

[Cartridge trap column]

This trap column does not decrease the theoretical plate number because of its very small dead volume. Not only
that, because it is a cartridge-type trap column, it is more economical

Column Structure 

[Non-sleeved type]

The chromatography tube is a fused silica capillary. The dead volume of the column is reduced through direct connection of the column with the chromatograph . This column is most suitable for the identification of phosphorylated peptides by LC/MS/MS because metallic parts are not used.

• Inner diameter: 0.075 mm and 0.1mm
• External diameter: 0.360 mm

[PEEK-sleeved type]

This column is easy to handle because it is a fused silica capillary with an outer sleeve of PEEK resin. Connection to MS is easy because connecters are attached to the column.

• Inner diameter: 0.075 mm–0.3 mm
• Connection: 1/16 inch stainless-steel connector