Immobilized Polysaccharide Columns
Daicel immobilized columns exhibit greatly enhanced robustness and remarkable stability. These innovative products allow chromatographers to employ essentially any organic solvent as mobile phase to develop methods for the most challenging separations. The immobilized columns are used for applications ranging from analytical method development to large-scale enantiomer resolutions.
Driven by the need for increased productivity in the lab, Daicel Corporation has introduced the first sub-2 µm immobilized chiral columns, CHIRALPAK IA-U, CHIRALPAK IB-U, CHIRALPAK IC-U and CHIRALPAK IG-U. The ultrafast separations provided by sub-2 µm columns speed-up initial drug discovery and enable rapid high-throughput screening and method development. These columns are designed to be used with Ultra-High Performance Liquid Chromatography (UHPLC) systems to achieve ultrafast and high-resolution of enantiomers.
- Normal phase: n-Hexane, IPA, EtOH
- Special mobile phase: Ethyl acetate, CH 3 Cl, THF, MTBE
- Reverse phase: H2O , IPA, MeOH
|Particle size of filler||CHIRALPAK® IA: 5 μm CHIRALPAK® IA-3: 3 μm CHIRALPAK® IA - U: 1.6 μm|
|pressure||The upper pressure limit of CHIRALPAK ® IA/CHIRALPAK ® IA-3 is 30MPa.
The upper pressure limit of CHIRALPAK ® IA-U is 70MPa.
|PH value||PH 2.0 to 9.0|
5~25℃ (reversed phase mode with pH>7)
|Store the solvent||CHIRALPAK ® IA: n-Hexane/Ethanol = 90/10 (v/v)
CHIRALPAK ® IA-3: n-Hexane/2-Propanol = 90/10 (v/v)
CHIRALPAK ® IA-U: n-Hexane/ 2-Propanol = 90/10 (v/v)
※ a package to protect the column core contains three pillars. The protection of the pillar core must be used in conjunction with the large cloak guard column (item No. 00011) .
Amylose tris (3,5-dimethylphenylcarbamate) immobilized to silica gel covalently bonded to the surface of silica gel
Silica gel surface covalently bonded amylose-tris (3,5-dimethylphenylcarbamate)
IA solvent-resistant chiral column, silica gel surface covalent bond with amylose-tris (3,5-dimethylphenylcarbamate).
Solvents use unrestricted, wide dissolution conditions, any solvent can be used as a mobile solvent, dissolved samples, chiral column regeneration.
Solvents that can be used, for example:
Positive phase: n-Hexane, IPA, EtOH
Special mobile phase: Ethyl acetate, CH 3 Cl, THF, MTBE
Reverse phase: H 2 O, IPA, MeOH
Country of OriginJapan
Internal Diameter (mm)4, 4.6, 2.1, 21, 20, 10, 30, 50
Column Length (mm)10, 50, 150, 250, 300, 100, 20
Particle size (µm)5, 10, 20
Stationary PhaseCHIRALPAK® IA, CHIRALPAK® IA SFC, CHIRALPAK® IA-10 SFC
TypeSemi-Preparative, Analytical, Guard Cartridges (x 3), Guard Semi-Preparative, Guard, Preparative, Guard Preparative