Daicel 25622 CHIRALPAK ® WH Ligand Exchange Analytical Guard HPLC Chiral Column, 4.6mm, 50mm, 10µm
CHIRALPAK ® WH
The surface of silica gel is covalently bonded to L-proline derivatives
USP L32 Specifies the filler
CHIRALPAK ® WH is a ligand exchange chiral column, and the surface of silica gel is covalently bonded to L-proline derivatives.
For the mobile phase of 0.1 ~ 2mM CuSO4 aqueous solution.
Ligand Exchange Chiral Stationary Phases
CHIRALPAK® WH / CHIRALPAK® MA(+) columns
The chiral stationary phases in these columns are made of amino acids and its derivatives coated or bonded to silica supports ( with a particle size of 10μm for WH and 3μm for MA (+) ) . Since these columns are ligand-exchange type columns, the standard mobile phase to use is an aqueous solution of CuSO4 (0.1 to 2mM) . These columns can tolerate organic moditifiers such as Methanol and Acetonitrile according to the specifications in the instruction manual.
Parameters
| Particle size of filler | 10 μm |
|---|---|
| pressure | In order to extend the service life of the chiral column, it is recommended to keep the pressure below 10 MPa |
| temperature | 0 ~ 50 ℃ |
| Store the solvent | 0.25 mM aqueous solution of CuSO4 100% |
In the presence of chiral compounds with low solubility in the range of available mobile phases, it is recommended to use a bonded chiral column (solvent-resistant chiral column) and try other mobile phase conditions.