Shodex F6709558 RSpak RP-18 G Polymeric Reversed Phase HPLC Guard Columns - Styrene divinylbenzene copolymer, Particle size (µm) 6, Column Length 10mm, 4.6mm Column ID
Specifications
Product Acetonitrile | 0 to 100% |
Product In-column solvent | H₂O/CH₃CN (5/95) |
Product Max. Pressure (MPa) | 22 |
Product Max. Usable Flow Rate (mL/min) | 1.5 |
Product Methanol | 0 to 100% |
Product Particle size, um | 6 |
Product pH range | 1 to 13 |
Product Plate number (TP/Column) | >5,000 |
Product Pore Size Average (Å) | 450 |
Product Salt conc. (M) | < 0.5 |
Product Size (I.D. x Length, mm) | 4.6 x 150 |
Product Temperature Range (°C) | RT to 50 |
Product THF | 0 to 50% |
Product Toluene Chloroform etc. | 0 to 50% |
Product Water solution | 0 to 100% |
Reversed Phase Chromatography (RP)
- Separation is based on the partition equilibrium between stationary phase and mobile phase.
- The polarity of the stationary phase is lower than that of the mobile phase.
- Typically the mobile phase contains a mixture of organic solvents (methanol, acetonitrile, or THF) and aqueous solvents (water or buffer).
- Using the lower polarity mobile phase causes a faster elution.
RSpak RP18
- Large pore size is suitable for the analysis of proteins and peptides (biomolecules)
- Corresponds USP LP21
Standard Proteins (3) (RP18-415)
RSpak RP18-415 (a column for reversed phase chromatography) can analyze a variety of proteins with good recovery. This column is suitable for fast analysis at high flow rates since the maximum working pressure is 22Mpa.
Recovery of Proteins | |
---|---|
1. Ribonuclease A | 93% |
2. Insulin | 98% |
3. Cytochrome c | 100% |
4. Lysozyme | 100% |
5. BSA | 98% |
6. Myoglobin | 108% |
7. Ovalbumin | --- |
Column : Shodex RSpak RP18-415 (4.6mmI.D. x 150mm) Eluent : (A); 0.1% TFA aq./CH3CN=99/1 (B); 0.1% TFA aq./CH3CN=5/95 Linear gradient: 0 to 25min, 20% (B) to 60% (B) Flow rate : 1.0mL/min Detector : UV(220nm) Column temp. : Room temp.