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Hamilton 79432 Cation Exchange HPLC Column, HC-40 (Ca2+) 10-15 µm 7.8 x 305 mm
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Hamilton 79432 Cation Exchange HPLC Column, HC-40 (Ca2+) 10-15 µm 7.8 x 305 mm

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In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples. The stronger the positive (+) charge on the sample, the stronger it will be attracted to the negative charge on the stationary phase, and thus the longer it will take to elute. The mobile phase is an aqueous buffer, where both pH and ionic strength are used to control elution time. Ion chromatography can employ harsh conditions requiring mobile phases that are at very high pH limits (> 11). Temperatures well above the normal operating conditions where silica materials fail can also be used.

Specifications

Stationary Phase HC-40 (Ca2+)
Mode Cation Exchange
USP Classification USP L19
Packing Material Type PSDVB/Sulfonic Acid
Particle Size 10-15 µm
Pore Size Gel-Type
Format Column
Hardware Inner Diameter 7.8 mm
Hardware Length 305 mm
Hardware Material Stainless Steel
Max. Pressure 1000
Exchange Capacity 5 meq/gm
Mobile Phase Limits 100% Water
Buffer Strength Water
Temp Limits 25-90°C
Restoration Flush with 1% calcium chloride at 0.1 mL/min overnight
Compound Classification Sugar oligomers up to DP8
UOM 1 EACH
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Separate compounds through size exclusion

HC-40 columns separate compounds through size exclusion. The 4% cross-linked HC-40 uses size exclusion as the primary mechanism of separation. The higher carbohydrate oligomers elute first while the smaller di- and monosaccharaides elute later.

 

Reversed Phase Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-1 PSDVB* N/A 100 Å 5-85 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-C18 C18 bonded to PSDVB* N/A 100 Å 5-85 °C pH 1 – 13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-3 PSDVB* N/A 300 Å 5-85 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-h5 Fluorinated PSDVB* N/A 300 Å 5-85 °C pH 1 – 13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
HxSil C18 Silica N/A 110 Å 5-60 °C pH 1.5 – 10. 0-100% aqueous, organic modifier N/A 6,000 psi
HxSil C8 Silica N/A 110 Å 5-60 °C pH 1.5 – 10. 0-100% aqueous, organic modifier N/A 6,000 psi

 

Anion Exchange Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-X100 PSDVB* with Tri- methyl ammonium Exchanger 0.19meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-X110 PSDVB* with Tri-methyl ammonium Exchanger 0.11meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-X500 Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride) 1.6meq/gm Superficially porous pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-2.0N 5,000 psi
PRP-X600 Poly (di-methyl amido-propyl meth-acrylamide) 1.6meq/gm Superficially porous pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-1.0N 5,000 psi
RCX-10 PSDVB* with Tri-methyl ammonium Exchanger 0.35meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-1.0N 5,000 psi
RCX-30 PSDVB* with Tri-methyl ammonium Exchanger 1.0meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-1.0N 5,000 psi

 

Cation Exchange Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-X200 PSDVB* Sulfonate Exchanger 35ueq/gm 100 Å 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-X400 PSDVB* Sulfonate Exchanger 2.5meq/gm N/A 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-5.0N 5,000 psi
PRP-X800 PSDVB* Itaconate Exchanger 1.6meq/gm 100 Å 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
HC-40 Calcium PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water Water 1,000 psi
HC-75 Calcium PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water 0-40% Acetonitrile Water 400 psi
HC-75 Hydrogen PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water 0-40% Acetonitrile Water 400 psi
HC-75 Lead PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water 0-40% Acetonitrile Water 400 psi

 

Ion Exclusion Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-X300 PSDVB* Sulfonate Exchanger 0.17meq/gm 100 Å 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi

*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C

 

An HPLC column has a finite lifetime.  It is recommended that you routinely monitor a column's retention characteristic and performance using standards.  If you plan to store the column for a prolonged period of time, use a mobile phase that inhibits microbial growth (a mobile phase containing sodium azide or high concentrations of methanol, acetonitrile, etc.).  For optimum performance and column life, follow these recommendations.

  1. Routinely monitor the column's performance.
  2. Switch only between miscible mobile phases.
  3. Avoid precipitating salts in the column.
  4. Use filtered and degassed mobile phases.
  5. Do not allow the column to dry out. Keep it tightly capped when not in use.
  6. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth.
  7. Use the column in the direction indicated on the label. An unusually high operating pressure may indicate a plugged inlet frit and may be cleared by reversing flow through the column for 10-20 mL.
  8. Use Hamilton guard columns to remove particulate matter or impurities that may permanently bind to the analytical column.

Restoring Column Performance
The procedures listed below may restore the performance of a column that has become fouled, but the procedures will not improve the performance of a column that is damaged or beyond its useful life. Nor can all stationary phases be restored. The unique supports in PRP-X500 and PRP-X600 columns make it impossible to restore performance with a washing procedure. For technical assistance regarding these columns, please Contact HPLC Support. NOTE: Before performing a restoration procedure, always make sure that the mobile phases are miscible and that precipitation will not occur. If necessary, wash the column with a suitable intermediate solvent before changing over to the new mobile phase. A minimum of five column volumes of intermediate solvent should be used.

Column Restoration Procedures
Follow the appropriate restoration procedure listed below using a flow rate of 1.0 or 2.0 mL/min. unless otherwise specified. Evaluate column performance using the test mix and conditions listed on the Performance Test Report shipped with each column.

 

 

 

Column Restoration Procedures

Packing Material Name

 

Restoration Procedure

PRP-C18

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes.
PRP-1  

PRP-3

 

PRP-h5

 

HxSil C8

 

HxSil C18

 

PRP-X100

Pump approximately 50 mL of methanol with 1% 6 N nitric acid

PRP-X200

Inject several times with 100 µL of 1 N nitric acid

PRP-X300

 

PRP-X400

Inject Several times with 100 µL of 0.1 M potassium EDTA

RCX-10

Flush with 50 mL 0.1 N sodium hydroxide

RCX-30

Flush with 150 mL 0.1 N sodium hydroxide

HC-40

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight

HC-75

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight

 

H+      Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight

 

Pb2+  Flush with 1% lead nitrate at 0.1 mL/min overnight

 

 

    • SKU
      79432
  • Manufacturer
    Hamilton Company
  • Chromatography HPLC or GC
    • Particle size (µm)
      10-15
    • Internal Diameter (mm)
      7.5
    • Length (mm)
      305
    • Temperature range (°C)
      25-90°C
    • Packing Phase
      HC-40 (Ca2+)