Hamilton 32908 Analytical Guard Cartridge Holder, Stainless Steel

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Specifications

Format Guard Cartridge Holder
Guard Column Size Analytical
Hardware Material Stainless Steel
UOM 1 EACH
Call 01463 830401 For Any Assistance

Hamilton PRP-X300 columns offer an easy, rapid way to separate closely related alcohols and organic acids. The sulfonated poly(styrene-divinylbenzene) support separates samples via a mixed mode mechanism. Separation on the PRP-X300 is accomplished by three modes:

  • Hydrogen Bonding—The attraction and retention of sample compounds by the negatively charged sulfonate group.
  • Reversed-Phase—The interaction and retention of the sample compounds by the non-polar polymeric support.
  • Ion Exclusion—The process in which ionized samples are excluded from the pores of the support and elute first, while the weakly ionized and nonionic compounds elute later.

 

Reversed Phase Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-1 PSDVB* N/A 100 Å 5-85 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-C18 C18 bonded to PSDVB* N/A 100 Å 5-85 °C pH 1 – 13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-3 PSDVB* N/A 300 Å 5-85 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-h5 Fluorinated PSDVB* N/A 300 Å 5-85 °C pH 1 – 13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
HxSil C18 Silica N/A 110 Å 5-60 °C pH 1.5 – 10. 0-100% aqueous, organic modifier N/A 6,000 psi
HxSil C8 Silica N/A 110 Å 5-60 °C pH 1.5 – 10. 0-100% aqueous, organic modifier N/A 6,000 psi

 

Anion Exchange Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-X100 PSDVB* with Tri- methyl ammonium Exchanger 0.19meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-X110 PSDVB* with Tri-methyl ammonium Exchanger 0.11meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-X500 Poly(meth-acryl amido-propyl Trimethyl-ammonium chloride) 1.6meq/gm Superficially porous pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-2.0N 5,000 psi
PRP-X600 Poly (di-methyl amido-propyl meth-acrylamide) 1.6meq/gm Superficially porous pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-1.0N 5,000 psi
RCX-10 PSDVB* with Tri-methyl ammonium Exchanger 0.35meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-1.0N 5,000 psi
RCX-30 PSDVB* with Tri-methyl ammonium Exchanger 1.0meq/gm 100 Å pH Dependent** pH 1-13. 0-100% aqueous, organic modifier 0.0-1.0N 5,000 psi

 

Cation Exchange Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-X200 PSDVB* Sulfonate Exchanger 35ueq/gm 100 Å 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
PRP-X400 PSDVB* Sulfonate Exchanger 2.5meq/gm N/A 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-5.0N 5,000 psi
PRP-X800 PSDVB* Itaconate Exchanger 1.6meq/gm 100 Å 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi
HC-40 Calcium PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water Water 1,000 psi
HC-75 Calcium PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water 0-40% Acetonitrile Water 400 psi
HC-75 Hydrogen PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water 0-40% Acetonitrile Water 400 psi
HC-75 Lead PSDVB* Sulfonate Exchanger 5meq/gm Gel-type 25-90 °C 100% Water 0-40% Acetonitrile Water 400 psi

 

Ion Exclusion Columns

Packing Name Support Material Exchange Capacity Pore Size Temp. Limits Mobile Phase Limits Buffer Strength Maximum Pressure
PRP-X300 PSDVB* Sulfonate Exchanger 0.17meq/gm 100 Å 5-60 °C pH 1-13. 0-100% aqueous, organic modifier 0.0-0.5N 5,000 psi

*PSDVB is Poly(styrene-divinylbenzene)
**pH 1-7.9 Temp. 5-60 °C; pH 8-13 Temp. 5-30 °C

 

An HPLC column has a finite lifetime.  It is recommended that you routinely monitor a column's retention characteristic and performance using standards.  If you plan to store the column for a prolonged period of time, use a mobile phase that inhibits microbial growth (a mobile phase containing sodium azide or high concentrations of methanol, acetonitrile, etc.).  For optimum performance and column life, follow these recommendations.

  1. Routinely monitor the column's performance.
  2. Switch only between miscible mobile phases.
  3. Avoid precipitating salts in the column.
  4. Use filtered and degassed mobile phases.
  5. Do not allow the column to dry out. Keep it tightly capped when not in use.
  6. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth.
  7. Use the column in the direction indicated on the label. An unusually high operating pressure may indicate a plugged inlet frit and may be cleared by reversing flow through the column for 10-20 mL.
  8. Use Hamilton guard columns to remove particulate matter or impurities that may permanently bind to the analytical column.

Restoring Column Performance
The procedures listed below may restore the performance of a column that has become fouled, but the procedures will not improve the performance of a column that is damaged or beyond its useful life. Nor can all stationary phases be restored. The unique supports in PRP-X500 and PRP-X600 columns make it impossible to restore performance with a washing procedure. For technical assistance regarding these columns, please Contact HPLC Support. NOTE: Before performing a restoration procedure, always make sure that the mobile phases are miscible and that precipitation will not occur. If necessary, wash the column with a suitable intermediate solvent before changing over to the new mobile phase. A minimum of five column volumes of intermediate solvent should be used.

Column Restoration Procedures
Follow the appropriate restoration procedure listed below using a flow rate of 1.0 or 2.0 mL/min. unless otherwise specified. Evaluate column performance using the test mix and conditions listed on the Performance Test Report shipped with each column.

 

 

 

Column Restoration Procedures

Packing Material Name

 

Restoration Procedure

PRP-C18

Flush with 40:40:20 (ACN:IPA:H20) for 5-10 column volumes.

PRP-1  

PRP-3

 

PRP-h5

 

HxSil C8

 

HxSil C18

 

PRP-X100

Pump approximately 50 mL of methanol with 1% 6 N nitric acid

PRP-X200

Inject several times with 100 µL of 1 N nitric acid

PRP-X300

 

PRP-X400

Inject Several times with 100 µL of 0.1 M potassium EDTA

RCX-10

Flush with 50 mL 0.1 N sodium hydroxide

RCX-30

Flush with 150 mL 0.1 N sodium hydroxide

HC-40

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight

HC-75

Ca2+  Flush with 1% calcium chloride at 0.1 mL/min overnight

 

H+      Flush with 0.1 N sulfuric acid at 0.1 mL/min overnight

 

Pb2+  Flush with 1% lead nitrate at 0.1 mL/min overnight

 

 

    • SKU
      32908
  • Manufacturer
    Hamilton Company
  • Chromatography HPLC or GC
    • Packing Phase
      PRP-X300