- Acid Corrosive Cabinets
- Arabidopsis Chambers
- Air Compressors
- Anemometer
- Autoclaves
- Balances
- Baths
- Blenders
- BOD Measurement
- Bunsen Burners
- Calibration Weights
- Calorimeters
- Cell Disruptors Ultrasonic
- Cell Manipulation
- Centrifuges
- Chemical Oxygen Demand
- Chromatography Columns & Consumables
- Climate Chambers
- Circulating Coolers
- Cold Boxes
- Colony Counters
- Colorimeters and Photometers
- Conductivity Meters
- Cryogenics
- Density Meters
- Desiccator
- Dissolved Oxygen
- Dispersers
- Dry Block Heaters
- Electrophoresis
- Flocculator
- Freezer Dryers
- Fume Hood Safety Containments
- Furnances
- Glove Box
- Hazardous Storage Cabinets
- Gas Generators
- Heating Mantles & Tapes
- Humidity Cabinets
- Hotplates
- Ice Machines
- Incubators
- Kjeldahl Equipment
- Laboratory Air Drying Cabinet or Warming Cabinet
- Laboratory Glasswashers and Accessories
- Liquid Handling Products
- Melting Point
- Microbiological Safety Cabinets
- Microscopes
- Milling
- Mixers
- Mixing and Storage Vessels
- Multi Parameters Meters
- Organisers and Bins
- Ovens
- PCR Workstation and UV Cabinets
- Plant Growth Cabinets
- Pellet Presses
- Personel Protective Equipment
- pH Measurement
- Pumps
- Refractometers
- Refrigeration - Refrigerators and Freezers
- Rheometers
- Rotators
- Rotary Evaporator
- Shakers
- Sieve Shakers
- Spare Parts
- Specialist Application Products
- Spectrophotometers
- Stirrers
- Stomachers
- Thermal Cyclers
- Toxic Substance Cabinets
- Titration
- Turbidity
- Ultrasonic Cleaners
- Ultra Violet Products
- Vacuum Chambers
- Vibratory Feeder
- Viscometers
- Water Purification Systems
- Water Stills
- Demonstration Stock
Daicel 38712 CHIRALPAK® RSA Rat Serum Albumin Protein Based Chiral Analytical HPLC Column, 4mm, 50mm, 5µm
We recommend the use of a guard cartridge, in order to protect the analytical column from impurities with high affinity and particulate impurities. The guard cartridge should be replaced regularly; otherwise the column performance can be affected. This is of special importance in bioanalysis.
Operating Conditions
- The column is filled with 10% 2-PrOH in water.
- Start at a low flow rate (column flushing-1) and maintain this flow for 2 min.
- Increase the flow rate (column flushing-2) and continue the washing for 10min.
- Equilibrate the column with the mobile phase to be used.
- Recommended flow rate is 0.25mL/min with a 2.0mm I.D. column; 0.5mL/min with a 3.0mm I.D. column; 0.9mL/min with a 4.0mm I.D. column
Temperature
The column should be used at room temperature or below
Mobile Phase Composition
- Buffer: Phosphate or acetate buffers should be used (0.01 to 0.1M)
- pH: The column can be used in the pH-range 5 to 7.
- Organic modifier: 2-Propanol and Acetonitrile are the most commonly used (less than 10%)
Samples
The recommended sample concentration is less than 0.1mg/mL with an injection volume of 5-20µL. If possible, dissolve the sample in the mobile phase. If the sample is insoluble in the mobile phase, add a higher concentration of the organic modifier. However, be aware of that too high organic modifier concentration might precipitate the buffer salts. Avoid dissolving the sample in pure organic solvent.
Do not inject unclear sample solutions or samples containing undissolved compounds