Daicel 34794 CHIRALPAK® HSA Protein Based Chiral Analytical HPLC Column, 2mm, 150mm, 5µm

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We recommend the use of a guard cartridge, in order to protect the analytical column from impurities with high affinity and particulate impurities. The guard cartridge should be replaced regularly; otherwise the column performance can be affected. This is of special importance in bioanalysis.

 

DescriptionCHIRALPAK® HSA 5µm
ManufacturerDaicel
Length150 mm
Internal Diameter2.0 mm
Particle Size5 µm
ModificationHSA
pH-RangepH = 2-7
Base MaterialSilica, spherical, porous
Packing brandCHIRALPAK®

 

CHIRALPAK ® HSA

Silica surface covalent bonding of human serum albumin

HSA is a protein-bonded chiral column with silica gel surface covalently bound to human serum albumin.

Suitable for inverting systems.

Suitable compounds isolated range:
strong acid, weak acid, zwitterionic and aprotic compounds.

 

Parameters

Filler particle sizeCHIRALPAK ® HSA: of 5 m
PHPH 5.0 to 7.0
temperature20 to 30 ° C
The solvent was storedThe H 2 O / 2-Propanol = 90/10 (V / V)
Precautions for storageIn order to prevent high temperature and lead to protein denaturation, damage to the separation of chiral column capacity, it is recommended to save in the refrigerator (4 ℃)
Call 01463 830401 For Any Assistance

CHIRALPAK HSA

  • The chiral selector is human serum albumin (HSA). The protein has been immobilized onto spherical 5 micrometer particles.
  • CHIRALPAK®HSA is especially suited for the separation of weak and strong acids, zwitterionic and nonprotolytic compounds.


CHIRALPAK® HSA can offer high enantioselectivity for compounds bearing carboxylic groups, including: - strong and weak acids - zwitter-ionic molecules - non-ionisable compounds (amides, esters, alcohols, sulfoxides, etc) For compounds of basic category, however, it is preferred to use CHIRALPAK® AGP and CHIRALPAK® CBH columns.

Scope: 
- an amine (primary amine, secondary amine, tris stage amine, four amine compound)
- an acid compound (a strongly acidic compound, a weakly acidic  compound)
- the aprotic compound (amides,  esters compounds, alcohols, sulfoxides of the  compounds, and the like)
- Acids and neutrals
- Excellent for hydrophilic acids

The chiral selector in this stationary phase is human serum albumin (HSA). The protein has been immobilized onto spherical 5μm particles. Enantiomers of preferentially acidic compounds can be resolved directly, without derivatization. The column is operated in the reversed phase mode. With the Chiral-HSA column, both racemic acids and amino acids can be resolved directly, without derivatization. 


Chromatographic Conditions

The column is operated in reversed phase mode: phosphate buffers (normally 0.01 to 0.1 M, pH 5-7) with the addition of less than 10% of an organic modifier such as 2-propanol, acetonitrile, methanol, or ethanol. Charged organic modifiers such as octanoic acid (1-5μM) may also be used. Enantio-selectivity and retention can be regulated by changing the mobile phase composition; ie: pH, buffer concentration and/or nature of the organic modifier.

Storage Conditions

The column should be used at room temperature or below. When the column is stored for long periods of time it is recommended to fill with 10% 2-propanol in distilled water and place it in the refrigerator.

Cleaning of the Column

If the column has been contaminated, wash the column over night with 10% 2-propanol in distilled water at a flow rate of 0.2mL/min  

    • SKU
      34794
    • Commodity Codes
      90279050
  • Manufacturer
    Daicel Corporation
  • Country of Origin
    Japan
  • Chromatography HPLC or GC
    • Particle size (µm)
      5
    • Internal Diameter (mm)
      2
    • Length (mm)
      150
    • Temperature range (°C)
      20 to 30 ° C
    • pH Range
      5-7
    • Packing Phase
      CHIRALPAK® HSA