Nacalai Tesque COSMOSIL 15781-91 CHiRAL 5A Immobilized Polysaccharide Derivative-Based HPLC Column, 10mm, 250mm, 5µm

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Features

  • Immobilized selectors can withstand many different solvents
  • Sharpen peaks with 3 µm particles
  • Preparative separations with 5 µm particles
  • Equivalent performance to columns currently on the market
  • Competitive price
Price Upon Application Or Discontinued

Conceptual Model

Conceptual Model

COSMOSIL CHiRAL Series

Packing MaterialCOSMOSIL CHiRAL 
3A, 5A
COSMOSIL CHiRAL 
3B, 5B
COSMOSIL CHiRAL 
3C, 5C
USP CodeL99

Base MaterialSilica gel
Particle Size3, 5 µm
Chiral SelectorAmylose tris (3,5-dimethylphenyl
carbamate)
Cellulose tris (3,5-dimethylphenyl
carbamate)
Cellulose tris (3,5-dichlorophenyl
carbamate)
StructureStructureStructure
Chiral Selector TypeImmobilized
Usable pH Range2-9

Immobilized Stationary Phase

High Resistance to Solvents

The COSMOSIL CHiRAL series uses a chiral selector that is chemically bonded to the silica gel, which results in high resistance to solvents. Tetrahydrofuran (THF) strips coated selectors from the stationary phase, reducing theoretical plates to less than 1/5 in our experiment. However, the same selectors bonded to the silica withstood repeated injections of THF.

Application

Conditions
Column size2.6 mm I.D. x 250 mmSampletrans-Stilbene oxide (0.5mg/ml)
Mobile phasen-Hexane / 2-Propanol = 90/10
Flow Rate1.0 ml/min
Temperature30°C
DetectionUV270nmInj.Vol.10 µl

Usable Solvents

Due to its immobilized selectors, various solvents are usable with COSMOSIL CHiRAL. A wide variety of solvents increases your selectivity options.

SolventImmobilizedCoated
Normal phaseReversed phaseNormal phase
n-Hexane◯*N/A◯*
n-HeptaneN/A
Methanol◯*◯*
Ethanol
2-Propanol (Isopropanol)
Acetonitrile◯*◯*
Tetrahydrofuran (THF)×
t-Butyl methyl etherN/A×
TolueneN/A×
ChloroformN/A×
Dichloromethane
(Methylene chloride)
N/A×
Ethyl acetateN/A×
WaterN/AN/A
Aqueous bufferN/AN/A

〇 Usable × Unusable
* Methanol / acetonitrile and n-hexane are not miscible with each other, so they should not be mixed for LC.
Elution strength: alcohols, THF >> chloroform > t-butyl methyl ether >> alkanes

Selectivity Depends on the Solvent

Column: COSMOSIL CHiRAL 3B, 4.6 mm I.D. x 250 mm
Sample: 1-Acenaphthenol

Application

Available in 3 µm Particles

Sharp Peaks

COSMOSIL CHiRAL columns are available in 3 µm particles, which yield sharper peaks than conventional 5 µm particles. Columns for preparative separations are available with 5 µm particles.

Comparison : 5µm/3 µm (1)

Application

Conditions
Column size4.6 mm I.D. x 250 mmSampleFlavanone (0.5mg/ml)Structure
Mobile phasen-Hexane / 2-Propanol = 90/10
Flow rate1.0 ml/min
Temperature30°C
DetectionUV 254 nmInj.Vol.1.0 µl
Comparison : 5µm/3 µm (2)

Application

Conditions
Column size4.6 mm I.D. x 250 mmSampleKetoprofen (0.3mg/ml)
Structure
Mobile phasen-Hexane / 2-Propanol / TFA = 90/10/0.1
Flow rate1.0 ml/min
Temperature30°C
DetectionUV 254 nmInj.Vol.1.0 µl

Moderate Backpressure

The COSMOSIL CHiRAL 3 series (3 µm particles) puts moderately higher pressure on the system compared to conventional 5 µm particles. This improves reproducibility for low-pressure solvents, such as hexane, that may otherwise cause pump irregularities. As shown in the figure below, increased 2-propanol concentration causes higher pressure. However, even at 50% (v/v), the pressure did not exceed 12 MPa. At the lowest concentration of 5% (v/v), the backpressure was 5.3 MPa. This range allows high flexibility in mobile phase composition and selectivity.

Table

Conditions
Packing materialCOSMOSIL CHiRAL 3A (3µm)
COSMOSIL CHiRAL 5A (5µm)
Column size4.6 mm I.D. x 250 mm
Mobile phaseHexane / 2-Propanol
(ratio as in figure)
Temperature30 °C
Flow rate1.0 ml/min

Compatible with SFC

COSMOSIL CHiRAL columns are compatible with SFC and exhibit similar performance compared to HPLC.

trans-Stilbene oxide

Condition
ColumnCOSMOSIL CHiRAL 3A
Column Size4.6 mm I.D. x 250 mm
Mobile Phasen-Hexane / 2-Propanol = 90/10
Flow Rate1.0 ml/min
Temperature30°C
DetectionUV254nm
Sampletrans-Stilbene oxide (2.0mg/ml)
Inj. Vol.1.0 µl

Condition
ColumnCOSMOSIL CHiRAL 3A
Column Size4.6 mm I.D. x 250 mm
Mobile PhaseA: CO2 
B: 2-Propanol 
B conc. 10%
Flow Rate3.0 ml/min
PBR10 MPa
Temperature40°C
DetectionUV254nm
Sampletrans-Stilbene oxide (2.0mg/ml)
Inj. Vol.2.0 µl

1-Phenoxy-2-propanol

Condition
ColumnCOSMOSIL CHiRAL 3C
Column Size4.6 mm I.D. x 250 mm
Mobile Phasen-Hexane / 2-Propanol = 90/10
Flow Rate1.0 ml/min
Temperature30°C
DetectionUV270nm
Sample1-Phenoxy-2-propanol (1.0mg/ml)
Inj. Vol.1.5 µl

Condition
ColumnCOSMOSIL CHiRAL 3C
Column Size4.6 mm I.D. x 250 mm
Mobile PhaseA: CO2 
B: 2-Propanol 
B conc. 10%
Flow Rate3.0 ml/min
PBR10 MPa
Temperature40°C
DetectionUV270nm
Sample1-Phenoxy-2-propanol (1.0mg/ml)
Inj. Vol.3.0 µl

Column Selection

COSMOSIL CHiRAL offers 3 different chiral selectors that, together, have a high probability of separating your sample. Of 28 samples in our test, 27 pairs of enantiomers were fully separated. For samples that do not separate easily with any column, please adjust the type and concentration of solvents in your mobile phase.

trans-Stillbene oxide
StructureHexane / 2-Propanol= 90 / 10
Complete Separation

Doble ring

Application
Complete Separation

Application
Complete SeparationApplication
Sample, mobile phaseCOSMOSIL CHiRAL ACOSMOSIL CHiRAL BCOSMOSIL CHiRAL C
2-(2,4-Dichlorophenoxy)
propionic acid
StructureHexane / 2-Propanol / TFA= 95 / 5 / 0.1
Complete Separation

Doublering

Application
ApplicationComplete SeparationApplication
1-(1-Naphthyl)ethanol
StructureHexane / 2-Propanol= 90 / 10
ApplicationComplete Separation

Doublering

Application
Complete SeparationApplication
Nicardipine
StructureHexane / 2-Propanol / Diethylamine= 90 / 10 / 0.1
ApplicationApplicationComplete Separation

Doublerings

Application
Chiral selector hit rate*(n = 28)60.70%67.90%71.40%

* Complete separation (hit) is defined as the two enantiomer peaks having resolution (RS) greater than or equal to 1.5.
The best separation for each sample is marked with double rings.

    • SKU
      15781-91
  • Manufacturer
    Nacalai Tesque Inc
  • Chromatography HPLC or GC
    • Particle size (µm)
      5
    • Internal Diameter (mm)
      10
    • Length (mm)
      250
    • pH Range
      2-9
    • Packing Phase
      COSMOSIL CHiRAL Series