Nacalai Tesque 12395-51 COSMOSIL PBr SFC Column, 4.6mm, 250mm, 5µm

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Supercritical Fluid Chromatography (SFC) has become more attractive because it offers some advantages over HPLC, such as high speed, unique selectivity and environmentally friendly separations. Many conventional normal-phase stationary phases, such as diol, amino and cyano, have been used for SFC applications. However, these phases present limitations for separations. COSMOSIL SFC Columns have been developed to enhance the capability of SFC separations.

Price Upon Application Or Discontinued

COSMOSIL SFC Columns

Nacalai Tesque has developed columns specially designed for SFC in collaboration with Nacalai USA and Pfizer, Inc. Global R&D: COSMOSIL HP, PY (equivalent to 2-ethylpyridine) and Quinoline. In addition to these, our HPLC columns Cholester and PBr have been tested for use with SFC.

Packing MaterialHPPYQuinolineCholesterPBr
Particle Sizes3, 5 µm2.5 µm5 µm2.5 µm5 µm5 µm
Pore Sizes120 Å130 Å120 Å130 Å120 Å120 Å
Surface Area300 m2/g330 m2/g300 m2/g330 m2/g300 m2/g300 m2/g
StructureStationary PhaseStationary PhaseStationary PhaseStationary PhaseStationary Phase
Stationary 
Phase
3-Hydroxyphenyl
group
Pyridinyl groupQuinoline groupCholesteryl groupPentabromobenzyl group

Pharmaceutical Analysis

Each phase has different retention properties.

Imipramine

Conditions
Column size2.1 mm I.D. x 150 mmSampleImipramine (1mmol/l)
Mobile phaseA: CO2
B: 0.1% CH3COONH4 -Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Flow Rate0.8 ml/min
BPR10 MPa
Temperature40°C
DetectionUV260nmInj.Vol.2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Procaine

Conditions
Column size2.1 mm I.D. x 150 mmSampleProcaine (1mmol/l)

Mobile phaseA: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→60% (0→14min), 60% (14-17min)
Flow Rate0.8 ml/min
BPR10 MPa
Temperature40°C
DetectionUV280nmInj.Vol.2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

Comparison of Retention Behavior

The following three stationary phases were evaluated for their retention of hydrophilic, hydrophobic, acidic and basic compounds. COSMOSIL HP and PY elute hydrophobic compounds first and retain hydrophilic compounds longer, whereas PBr elutes in the reverse order, exhibiting high retention for hydrophobic compounds. HP had the longest retention for basic compounds.

Applications

Conditions
Column size4.6 mm I.D. x 150 mmFlow Rate0.8 ml/min
Mobile phaseA: CO2
B: 0.1% CH3COONH4 - Methanol
B conc. 0→50% (0→14min), 50% (14-17min)
BPR10 MPa
Temperature40°C
DetectionESI-MS/MS, MRM
Sample
  1. Uridine
  2. 3’ ,5’ -Dibenzyloxyacetophenone
  3. 4-Ethylbenzenesulfonic Acid
  4. Benzyltrimethylammonium
Sample conc.8 µmol/l
Inj.Vol.2.0 µl

Data courtesy of Kyushu University Medical Institute of Bioregulation Research Center for Transomics Medicine Division of Metabolomics

    • SKU
      12395-51
  • Manufacturer
    Nacalai Tesque Inc
  • Chromatography HPLC or GC
    • Particle size (µm)
      5
    • Internal Diameter (mm)
      4.6
    • Length (mm)
      250
    • Packing Phase
      COSMOSIL SFC Columns