We recommend the use of a guard cartridge, in order to protect the analytical column from impurities with high affinity and particulate impurities. The guard cartridge should be replaced regularly; otherwise the column performance can be affected. This is of special importance in bioanalysis.

Temperature 

The column should be used at room temperature or below

Mobile Phase Composition 

• Buffer: Phosphate or acetate buffers should be used (0.01 to 0.1M) 
• pH: The column can be used in the pH-range 5 to 7. 
• Organic modifier: 2-Propanol and Acetonitrile are the most commonly used (less than 10%)

Samples 

The recommended sample concentration is less than 0.1mg/mL with an injection volume of 5-20µL. If possible, dissolve the sample in the mobile phase. If the sample is insoluble in the mobile phase, add a higher concentration of the organic modifier. However, be aware of that too high organic modifier concentration might precipitate the buffer salts. Avoid dissolving the sample in pure organic solvent. 

Do not inject unclear sample solutions or samples containing undissolved compounds

Click On The Red Link To Visit - The Chiral Application Search, which  has been developed to help you find the best enantiomeric separation.

By searching structure and application you will be able to access over 2100 chiral separations some of which offer multiple separation conditions . We have included examples of CHIRALCEL^® / CHIRALPAK^® (Polysaccharide, Protein and Quinine / Quinidine) and CROWNPAK^® (Crown ether) columns.

In addition, the application data of certain generic API compounds which are registered with the “USP, EP and JP Pharmacopeia are included alongside suggested alternative conditions found by Daicel scientists.