CERI 612290 L-column ODS Micro HPLC Column, 0.2mm x 50mm, 5μm Particle Size, PEEK-Sleeved

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This column is easy to handle because it is a fused silica capillary with an outer sleeve of PEEK resin. Connection to MS is easy because connecters are attached to the column. 

  • Inner diameter: 0.075 mm–0.3 mm  
  • Connection: 1/16 inch stainless-steel connector


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L-column Micro is a nano/micro column with 0.075–0.3 mm I.D. It is a high-performance column that was manufactured by combining low-absorption packing materials, including L-column2 ODS, an originally developed packing technique and a column structure with a small dead volume.

Features    

  • The packing materials can be selected from L-column series including L-column2 ODS.
  • Highly sensitive analyses are possible because of the negligible adsorption of basic peptides and proteins. 
  • Stabilization time can be shortened and highly sensitive analyses are possible because of low column bleed. 
  • An inert fused silica capillary is used as the column body, with two kinds of column structures available.


 Proteome analysis 

BSA tryptic digest was analyzed using LC/MS/MS and the sample concentration and cover ratio was determined using L-column Micro and another brand. A higher cover ratio means that more amino acid sequences are read. L-column Micro shows advantage at all concentration levels, but excels at the lower levels . With superior end-capping and very high theoretical plate numbers per column, L-column Micro permits identification of many proteins and is the optimum choice for proteome analysis 

Low adsorption and high efficiency 

L-column Micro can separate many peptides because of the low-adsorption packing materials. This is effective for the identification of proteins. The column has a high theoretical plate number and high durability because it is packed homogeneously by a patented technique.

Trap column

In proteomic analysis using LC/MS/MS, the trap column is indispensable for increasing the injection volume. A small dead volume, low-adsorption property and high retention ability that traps target constituents are required effectively in a trap column.

    • SKU
      612290
  • Manufacturer
    Chemicals Evaluation and Research Institute (CERI)
  • Chromatography HPLC or GC
    • Particle size (µm)
      5
    • Internal Diameter (mm)
      0.2
    • Length (mm)
      50
    • Packing Phase
      L-column ODS